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  Porcine Parvovirus (PPV) antibody ELISA Test Kit More >> 

Time of Issue: Mar. 1, 2010 13:04:18
Period of Validity: Mar. 31, 2010 13:04:18
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Principle

            The test kit is the antigen coated microplate made through the
            inactivation, purification and concentration of the porcine
            parvovirus. In the test, the diluted contrast serum and sample are
            added, then incubate. If porcine parvovirus specific antibodies
            exist in the sample , it will be bound with the antigens on the
            microplate, Remove the unbound antibodies and other components by
            washing. Add IgG-HRP to specifically bind with the compound of
            antibody and antigen on the microplate. Then the unbound conjugates
            will be removed by washing. Then add the TMB substrate to the well,
            react with HRP to a blue product. Stop the reaction by adding HF
            solution, measure the OD630nm value.
            Specifications

            Reagents of the Test Kit
            1 Antigen coated microplate…………2 pieces (96 wells)
            2 Negative control serum (green lid)…………1 tube (1ml/tube)
            Positive control serum (yellow lid)……………1 tube
            (1ml/tube)
            3 Anti-pig IgG-HRP combination(yellow lid)……1 bottle
            (22ml/bottle)
            4 20× concentrated washing liquid (white lid)……1 bottle
            (30ml/bottle)
            5 Substrate A (red lid) …………1 bottle each
            (12ml/bottle)
            Substrate B (black lid)…………1 bottle each
            (12ml/bottle)
            6 Stop solution (blue lid) ……………1 bottle
            (12ml/bottle)
            7 Sample diluent (transparent lid)…………1 bottle
            (50ml/bottle)
            Storage: store 2-8℃, not frozen.
            Expiry date: 6 months



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