Principle

The test kit is the antigen coated microplate made through the inactivation, purification and concentration of the PRRSV. In the test, the diluted control serum and sample are added, then incubate. If porcine PRRSV specific antibodies exist in the sample , it will be bound with the antigens on the microplate, remove the unbound antibodies and other components by washing. Add IgG-HRP to specifically bind with complex of antibody-antigens on the microplate. Then the unbound enzyme conjugate (Anti-pig IgG-HRP combination) will be removed by washing. Then add the TMB substrate to the wells, react with HRP to generate a blue color. Stop the reaction by adding HF solution, measure the OD630nm value.

 

Reagents of the Test Kit

1  Antigen coated microplate………………………………………………...2 pieces (96 wells)

2  Negative control serum (green lid) ……………………………………….1 tube (1ml)

3  Positive control serum (red lid)……………………………………………1 tube (1ml)

4  Anti-pig IgG-HRP combination(yellow lid)……………………………….1 bottle (22ml)

5  20×concentrated washing buffer (white lid)……………………………….1 bottle (30ml)

6  Substrate A (red lid)/B (black lid)………………………………………...1 bottle each (12ml/bottle)

7  Stop solution (blue lid)……………………………………………………..1 bottle (12ml)

8  Sample diluent (transparent lid)……………………………………………1 bottle (50ml)

Specifications : 96 wells×2.

Expiry date: 6 months.

Storage: Storing at 2~8℃, in the dark.