1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Salbutamal in the pork , liver, urine. The coupling antigen is pre-coated on the micro-well stripes. The Salbutamal in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Salbutamal antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Salbutamal in it. This value is compared to the standard curve and the Salbutamal residues is subsequently obtained.

 

2. Technical specifications

Specification: 96wells /kit

Sensitivity: 0.5 ppb

Detection limit

Tissue0.5 ppb

Urine, serum0.5 ppb

Feed50 ppb

Recovery rate

Urine, serum90±10%

Tissue80±10%

Feed80±15%

Cross-reaction rate

Salbutamal100%

Terbutalin< 1%

Clenbuterol< 13%

Ractopamine< 1%