Brief

Avian influenza is an infectious disease of birds caused by type A strains of the influenza virus. H5N1 is a type of avian influenza virus (bird flu virus) that has mutated through antigenic drift into dozens of highly pathogenic varieties, but all currently belonging to genotype Z of avian influenza virus H5N1. Genotype Z emerged through reassortment in 2002 from earlier highly pathogenic genotypes of H5N1 that first appeared in China in 1996 in birds and in Hong Kong in 1997 in humans. The "H5N1 viruses from human infections and the closely related avian viruses isolated in 2004 and 2005 belong to a single genotype, often referred to as genotype Z." The avian influenza subtypes that have been confirmed in humans, ordered by the number of known human deaths, are: H1N1 caused Spanish flu, H2N2 caused Asian Flu, H3N2 caused Hong Kong Flu, H5N1, H7N7, H9N2, H7N2, H7N3, H10N7.

Avian Flu viruses H₅N₁ subtype Antibody ELISA kit is composed of the antigen coated microplate, enzyme conjugate (HRP goat-anti-chicken IgG), and other reagents. It applies the indirect ELISA principle to test the antibodies of Avian Flu viruses H5N1 subtype in the serum.

Usage

Avian Flu viruses H5N1 subtype Antibody ELISA kit is used to detect anitibody of Avian Flu viruse in the checken serum, assessment of immunity conditions against Avian Flu viruses the chicken farm; investigation of the epidemiology of the Avian Flu viruses.

Principle

The test kit is the antigen coated microplate made from the inactivated, purified and concentrated recombinant protein. In the test, the diluted control serum and sample are added, then incubate. If the sample exists Avian Flu viruses H5N1 sub-type antibodies, they will be bound with the antigens of the microplate, the unbound antibodies and other components are removed by washing. Next, add enzyme conjugate (anti-chicken IgG-HRP) to specifically bind to the compound of antibody-antigens of the microplate, the unbound enzyme conjugate will be removed by washing, add the TMB substrate into the wells, react and generate a blue color. At last, end the reaction by adding stop solution,read the absorbance value at  450/630nm.

Reagents of the Test Kit

1  Antigen coated microplate......................................................................... 2 pieces (96 wells)

2  Negative (green lid) control ................................................................................ 1 tube (1ml)

3  Positive (red lid) control...................................................................................... 1 tube (1ml)

4  Anti-chicken IgG-HRP combination(yellow lid)............................................ 1 bottle (22ml)

5  20×concentrated washing solution (white lid)............................................... 1 bottle (30ml)

6  Substrate A (red lid)/B (black lid)................................................ 1 bottle each (12ml/bottle)

7  Stop solution (blue lid)...................................................................................... 1 bottle (12ml)

8  Sample diluent (transparent lid)....................................................................... 1 bottle (50ml)

Specifications : 96 wells×2.

Expiry date: 6 months.

Storage: Storing at 2~8℃, in the dark.